validation and evaluation of the chemical nature of ingredients

The equipment used to validate ingredient synthesis includes:

FTIR
UV-VIS
Laser Low Angle Light scattering spectrometers
SPF UV Trans Spectrometer
Multiple HPLC's, GC's
Automated Titration Equipment
Molecular Weight determination
Viscometry

testing in vitro efficacy and safety of bioactive concentrates and products

OBI, in cooperation with TPI, operates a joint program to develop a library of potential bioactive ingredients classified as to their effects on gene expression after addition to keratinocytes, fibroblasts and melanocytes in human cell culture. These results are correlated with conventional ELISA, CyQuant and Soluble Protein Assays. This work is conducted with the following on site resources:

Cell and Tissue Laboratory
Microbiology Laboratory
qPCR Roche System (Roche) 42 and 82 gene arrays
Fluorescence Microplate Readers
UV / Vis Microplate Readers
CyQuant / Rate of Proliferation Enhancement
Extracellular Soluble Protein Enhancement
Alamar Blue / Cytotoxicity LD50
Elisa Kits (Defensin B, UVDNA damage, Oxidative DNA damage etc.)
UVA / UVB Lamp assembly
Visible, UV, Phase Contrast Photo Microscopy

specific efficacy test categories:

Anti-aging (cell turnover enhancement/ soluble protein production):
TPI has conducted qPCR gene array assays for the top 30 ingredients claimed for anti-agingon four cell types (keratinocytes, fibroblasts (37 yoff, 66 yoff), keratinocytes, andmelanocytes) as a reference library.

Pigmentation (pigment suppression/ selective pheomelannin production):
TPI has conducted qPCR gene array assays on the top 20 ingredients claimed forpigmentation/ anti-oxidant activity on melanocytes as a reference library.

Oxidation / UV DNA Repair:
Produce qPCR gene array assays on top 30 ingredients claimed for pigmentation / anti-oxidant activity on four cell types.

Owen Biosciences, Inc. 2015